The DOT Urine Specimen Collector Training — 2026 Edition provides comprehensive training in the procedures and responsibilities associated with conducting urine specimen collections for U.S. Department of Transportation (DOT) workplace drug-testing programs.
This course is based on 49 CFR Part 40 and the DOT Urine Specimen Collection Procedures Guidelines, revised June 2026, and is designed to help learners develop the knowledge required to properly conduct and document DOT urine specimen collections.
Throughout the course, learners will examine the collector’s role and qualification requirements, collection-site security and integrity, Federal Drug Testing Custody and Control Form (CCF) procedures, employee identification, specimen collection procedures, insufficient specimens, directly observed and monitored collections, refusals and unusual collections, collection errors, specimen packaging and shipment, recordkeeping, and collector qualification documentation.
The course also addresses the initial proficiency demonstration required under 49 CFR §40.33, including the five consecutive error-free mock collections required as part of initial collector qualification.
This course is appropriate for individuals who are preparing to perform DOT urine specimen collections or who need current training in DOT collection procedures, including:
Previous healthcare credentials or specimen-collection experience do not replace the applicable DOT collector qualification requirements.
Upon completion of the instructional course, learners should be able to:
The course includes:
10 instructional modules
Module knowledge checks
Practical collection scenarios
Official DOT reference materials
Federal CCF instruction
Collector compliance resources
Comprehensive final examination
Completion of the AHTI instructional course alone does not constitute completion of all requirements for initial qualification as a DOT urine specimen collector.
Under 49 CFR § 40.33, initial qualification includes successful completion of the required proficiency demonstration consisting of five consecutive error-free mock collections.
Learners should retain documentation of both their qualification training and required proficiency demonstrations.
AHTI provides certificates and documentation reflecting the specific training and proficiency components successfully completed by the learner.
The U.S. Department of Transportation does not approve, certify, or recommend private collector-training programs. Completion of this course should not be represented as a DOT-issued certification or endorsement.
AHTI’s program provides professional training designed around applicable DOT collector requirements. Collectors remain responsible for following current 49 CFR Part 40, current DOT guidance, applicable DOT agency regulations, and employer or service-agent procedures when conducting DOT-regulated collections.
49 CFR Part 40 — Procedures for Transportation Workplace Drug and Alcohol Testing Programs
DOT Urine Specimen Collection Procedures Guidelines — June 2026
Anagail Health Training Institute is exempt from authorization by the Georgia Nonpublic Postsecondary Education Commission (GNPEC) under O.C.G.A. § 20-3-250.3(a)(3).
All programs are non-credit and are offered for continuing education and professional development purposes only. Programs are intended for individuals with prior education, training, or experience in a related field. Completion of any program does not grant academic credit, state licensure, or professional certification.
Anagail Health Training Institute issues certificates of completion only. Continuing Education Units (CEUs), where applicable, are provided through third-party organizations, such as Activated Insights. Acceptance of CEUs is determined by the participant’s employer or licensing authority.
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Anagail Health Training Institute is a workforce and professional development division of Anagail Care Services LLC, providing continuing education and skill-based training for healthcare and community-based professionals.
